Effect of deacetylation degree on controlled pilocarpine release from injectable chitosan-g-poly(N-isopropylacrylamide) carriers (2024)

Luo, L. J., Huang, C. C., Chen, H. C., Lai, J. Y., & Matsusaki, M. (2018). Effect of deacetylation degree on controlled pilocarpine release from injectable chitosan-g-poly(N-isopropylacrylamide) carriers. Carbohydrate Polymers, 197, 375-384. https://doi.org/10.1016/j.carbpol.2018.06.020

Luo, Li Jyuan ; Huang, Chih Ching ; Chen, Hung Chi et al. / Effect of deacetylation degree on controlled pilocarpine release from injectable chitosan-g-poly(N-isopropylacrylamide) carriers. In: Carbohydrate Polymers. 2018 ; Vol. 197. pp. 375-384.

@article{b4bfe59fad394dba880a0f35f42cb733,

title = "Effect of deacetylation degree on controlled pilocarpine release from injectable chitosan-g-poly(N-isopropylacrylamide) carriers",

abstract = "Development of biodegradable thermogels as intracameral injectable carriers for ocular delivery of antiglaucoma medications can provide a better treatment modality with low dosing frequency than eye drop formulations. For the first time, this study investigates the effect of deacetylation degree (DD) of the polysaccharide component in chitosan-g-poly(N-isopropylacrylamide) (CN) carriers on controlled release of pilocarpine in the management of glaucoma. Our results showed that increasing the chitosan DD from 60.7% to 98.5% leads to enhanced biodegradation resistance of carrier and prolonged release profile of the drug. Significant DNA damage and caspase-3 activation could be detected in lens epithelial cell cultures exposed to CN made from highly deacetylated polysaccharides, indicating apoptosis-related cytotoxicity due to relatively high positive charge density of the graft copolymers. Postoperative outcomes demonstrated that long-term therapeutic efficacy in glaucomatous rabbits is governed by intraocular pressure changes in response to intracamerally administered pilocarpine-loaded CN, strongly suggesting the usefulness of deacetylation in this injectable drug delivery carrier.",

keywords = "Biodegradable thermogels, Chitosan-g-poly(N-isopropylacrylamide), Controlled pilocarpine release, Deacetylation degree, Injectable drug carrier",

author = "Luo, {Li Jyuan} and Huang, {Chih Ching} and Chen, {Hung Chi} and Lai, {Jui Yang} and Michiya Matsusaki",

note = "Publisher Copyright: {\textcopyright} 2018 Elsevier Ltd",

year = "2018",

month = oct,

day = "1",

doi = "10.1016/j.carbpol.2018.06.020",

language = "英语",

volume = "197",

pages = "375--384",

journal = "Carbohydrate Polymers",

issn = "0144-8617",

}

Luo, LJ, Huang, CC, Chen, HC, Lai, JY & Matsusaki, M 2018, 'Effect of deacetylation degree on controlled pilocarpine release from injectable chitosan-g-poly(N-isopropylacrylamide) carriers', Carbohydrate Polymers, vol. 197, pp. 375-384. https://doi.org/10.1016/j.carbpol.2018.06.020

Effect of deacetylation degree on controlled pilocarpine release from injectable chitosan-g-poly(N-isopropylacrylamide) carriers. / Luo, Li Jyuan; Huang, Chih Ching; Chen, Hung Chi et al.
In: Carbohydrate Polymers, Vol. 197, 01.10.2018, p. 375-384.

Research output: Contribution to journal › Journal Article › peer-review

TY - JOUR

T1 - Effect of deacetylation degree on controlled pilocarpine release from injectable chitosan-g-poly(N-isopropylacrylamide) carriers

AU - Luo, Li Jyuan

AU - Huang, Chih Ching

AU - Chen, Hung Chi

AU - Lai, Jui Yang

AU - Matsusaki, Michiya

N1 - Publisher Copyright:© 2018 Elsevier Ltd

PY - 2018/10/1

Y1 - 2018/10/1

N2 - Development of biodegradable thermogels as intracameral injectable carriers for ocular delivery of antiglaucoma medications can provide a better treatment modality with low dosing frequency than eye drop formulations. For the first time, this study investigates the effect of deacetylation degree (DD) of the polysaccharide component in chitosan-g-poly(N-isopropylacrylamide) (CN) carriers on controlled release of pilocarpine in the management of glaucoma. Our results showed that increasing the chitosan DD from 60.7% to 98.5% leads to enhanced biodegradation resistance of carrier and prolonged release profile of the drug. Significant DNA damage and caspase-3 activation could be detected in lens epithelial cell cultures exposed to CN made from highly deacetylated polysaccharides, indicating apoptosis-related cytotoxicity due to relatively high positive charge density of the graft copolymers. Postoperative outcomes demonstrated that long-term therapeutic efficacy in glaucomatous rabbits is governed by intraocular pressure changes in response to intracamerally administered pilocarpine-loaded CN, strongly suggesting the usefulness of deacetylation in this injectable drug delivery carrier.

AB - Development of biodegradable thermogels as intracameral injectable carriers for ocular delivery of antiglaucoma medications can provide a better treatment modality with low dosing frequency than eye drop formulations. For the first time, this study investigates the effect of deacetylation degree (DD) of the polysaccharide component in chitosan-g-poly(N-isopropylacrylamide) (CN) carriers on controlled release of pilocarpine in the management of glaucoma. Our results showed that increasing the chitosan DD from 60.7% to 98.5% leads to enhanced biodegradation resistance of carrier and prolonged release profile of the drug. Significant DNA damage and caspase-3 activation could be detected in lens epithelial cell cultures exposed to CN made from highly deacetylated polysaccharides, indicating apoptosis-related cytotoxicity due to relatively high positive charge density of the graft copolymers. Postoperative outcomes demonstrated that long-term therapeutic efficacy in glaucomatous rabbits is governed by intraocular pressure changes in response to intracamerally administered pilocarpine-loaded CN, strongly suggesting the usefulness of deacetylation in this injectable drug delivery carrier.

KW - Biodegradable thermogels

KW - Chitosan-g-poly(N-isopropylacrylamide)

KW - Controlled pilocarpine release

KW - Deacetylation degree

KW - Injectable drug carrier

UR - http://www.scopus.com/inward/record.url?scp=85048256430&partnerID=8YFLogxK

U2 - 10.1016/j.carbpol.2018.06.020

DO - 10.1016/j.carbpol.2018.06.020

M3 - 文章

C2 - 30007625

AN - SCOPUS:85048256430

SN - 0144-8617

VL - 197

SP - 375

EP - 384

JO - Carbohydrate Polymers

JF - Carbohydrate Polymers

ER -

Luo LJ, Huang CC, Chen HC, Lai JY, Matsusaki M. Effect of deacetylation degree on controlled pilocarpine release from injectable chitosan-g-poly(N-isopropylacrylamide) carriers. Carbohydrate Polymers. 2018 Oct 1;197:375-384. doi: 10.1016/j.carbpol.2018.06.020